ASTM E1838-2010 使用成人用指垫测定卫生洗手和擦手剂的病毒清除效果的标准试验方法

作者:标准资料网 时间:2024-05-13 16:54:56  浏览:9289   来源:标准资料网

【英文标准名称】:StandardTestMethodforDeterminingtheVirus-EliminatingEffectivenessofHygienicHandwashandHandrubAgentsUsingtheFingerpadsofAdults
【原文标准名称】:使用成人用指垫测定卫生洗手和擦手剂的病毒清除效果的标准试验方法
【标准号】:ASTME1838-2010
【标准状态】:现行
【国别】:美国
【发布日期】:2010
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:E35.15
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:adenovirus;cellculture;cytotoxicity;eluent;fingerpads;hepatitisAvirus;hygienichandwashing;hygienichandrubagent;infectioncontrol;influenzavirus;invivotesting;microbicidalsoap;rhinovirus;rotavirus;
【摘要】:Thisinvivoprocedureisdesignedtotesttheabilityofhygienichandwashandhandrubagentstoreducelevelsofselectedinfectiousvirusesfromexperimentallycontaminatedfingerpadsofadults.Sincethetwothumbpadsandalleightfingerpadscanbeusedinanygiventest,itallowsfortheincorporationofinputviruscontrol(two),virusremainingviableaftertheinoculumhasbeenallowedtodry(two),viruseliminatedaftertreatmentwithacontrolorreferencesolution(two),anduptofourreplicatestoassessthevirus-eliminatingefficiencyofthesubstanceundertest.Nomorethan100x03BC;Lofthevirussuspensionarerequiredtocompleteonetest.Theresultsoftestingwiththistestmethodmayformthebasisforfurthertestsusingasuitablewhole-handtestprotocol(forexample,TestMethodE2011).Thistestmethodisdesignedtobeperformedbyatrainedindividual,whoisresponsibleforchoosingtheappropriatehostsystemforthetestvirusandapplyingthetechniquesnecessaryforpropagationandmaintenanceofhostandtestvirus.Forareferencetext,refertoLennetteetal(8).Whereasthemethoddescribedhererelatestotestingwithvirusesofhumanorigin,itcanbereadilyadaptedtoworkwithanimalpathogenicvirusesaswellasbacteriophages.Standardmethodsforworkingwithbacteria(TestMethodE2276)andfungi(TestMethodE2613)arealsoavailable.Infectiousmicroorganismsleftonhandsafterwashingcanbereducedfurtherbydryingthewashedhandswithpaper,cloth,orwarmair(9).Astepforthedryingoffingerpadsafterexposuretothecontrolortestsubstance,therefore,hasnotbeenincludedtoavoidvirusremovalbythedryingprocessitself.Thistestmethodisnotmeantforusewithsurgicalhandscrubsorpreoperativeskinpreps.Thelevelofviablevirusoneachfingerpadafterthedryingoftheinoculumshouldnotbelessthan104infectiousunitswhichwouldpermitthedetectionofuptoa4log10reductionintheinfectivitytiterofthevirusbythetestsubstanceundertheconditionsofthistestmethod.1.1Humanskinisnotknowntocarryvirusesasapartofitsresidentmicrobiota.Handstransientlycontaminatedwithvirusescan,however,actasvehiclesforthespreadofmanytypesofviralinfections.Handhygieneismeanttoreducetheloadofvirusesandothertransientmicroorganismsonhands,therebyreducingtheriskofdiseasetransmission.Suchreductionsinthevirusloadmaybeduetoacombinationofvirusinactivationandmechanicalremovalofinfectiousvirusfromtheskin.1.2Thistestmethodisdesignedtodeterminethecomparativevirus-eliminatingeffectivenessofmicrobicidalornon-microbicidalformulations.Thistestmethodisnotmeantforusewithsurgicalhandscrubsorpreoperativeskinpreps.Note18212;Thetestmethodshouldbeperformedbypersonswithtraininginvirologyinfacilitiesdesignedandequippedforworkwithinfectiousagentsatbiosafetylevel2(6).1.3ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddet......
【中国标准分类号】:Y43
【国际标准分类号】:11_080_01
【页数】:7P.;A4
【正文语种】:英语